I found two bits of info that might help.
BK: 17th Century Isle of Wight by Boddie
James Knott, Sr, aged 23, came over on the George and settled in Accomac in 1617. He owned land there in 1632 and he and wife Elinor sold this land June 11, 1634. (Order Bk, 1, Folio 37, Northampton.) March 24, 1635, James Knott patented 1200 acres in Nansemond for the transportation of himself, wife Elinor and 21 other persons. James Knott was probably one of those Puritans who joined in the trek to Maryland for he patented land there in 1652 but died soon afterwards. As "James Knott of Nansemond" he made his will there Sept. 4, 1651, and same was probated 13 May 1653. He mentioned three sons in his will but did not mention Francis and James. The records of St. Mary's county show that he conveyed land to his son Francis before his death. Probably on leaving Nansemond he likewise conveyed land to James, for the Quit Rent Rolls of Nansemond show that James was holding 1050 acres there in 1704, which was probably the same land James Sr was granted in 1635. Descendants of James Jr moved to North Carolina.
Bk: Early Virginia Families Along the James River. by Foley Vol III
There are several patents for a Wm. Knott mentioned but I thought this one would help you.
William Knott, 312 Acres, Surry Co 28 Mar 1666, p. 482 (land patents). 112 acres on south side of James River on south side of upper Chipoake Creek, bounded NW on land of Edward Oliver, N upon Wm. Thomas, E on George Gibson & SE on Mr. Fisher; 200 acres on south side of said River, Wly. on Jeremiah Clements, NW on Edward Oliver, Nly on Wm. Thomas, George Gibson & Edward Minter, Ely. on Wm. Gapins land & Mr. Thomas Busbie and SE on Mr. Richard Hill. 112 acres granted to William Knott dec'd, 20 Nov. 1653 & 200 acres in said Knotts name 14 Oct 1657 & now due him as a son and heire of said William. Trans. of 4 persons: Peter Bray, Wm. Hawking, Sarah Willis, Mary Jones.
According to this.....looks like William was a son of a William.
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